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KMID : 0361619930280010435
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Journal of the Korean Orthopaedic Association
1993 Volume.28 No. 1 p.435 ~ p.444
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An Experimental Study for Activities of Enzymes Involed in Nucleic Acid Degradation in Patients with Osteosarcoma
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김광희
김성준/김태승/최영준/고재경
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Abstract
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In order to investigate possible roles of enzymes involved in nucleic acid degradation in tumorigenesis of osteosarcoma, the activities of acid deoxyribonuclease (DNase) and neutral ribonuclease (RNase) in osteosarcoma tissue were determined.
Neutral
RNases were isolated and analyzed by ion exchange column chromatography to see whether the enzymes unique to the tumor were present. The activity of neutral RNase in serum of patient with osteosarcoma was also determined to find out whether the
serum
enzyme level could be used as diagnostic or therapeutic marker.
1. The activities of nucleases in osteosarcoma tissue were significantly increased and the positive rates were observed to be over 80% in acid DNase and 90 in neutral RNase.
2. The activity of serum neutral RNase in patient with osteosarcoma was increased by 77% (p<0.05) and the positive rates was considerably high.
3. neutral RNases in osteosarcoma tissue were separated by DEAE-cellulose column chromatography into five peaks, two of them were revealed to be unique to the tumor, and one of the peak RNases (peak V) was further separated by phosphocellulose
column
chromatography into three peaks, two of them appeared to be specific to osteosarcoma.
The results indicate that the nucleases could be use as biochemical and diagnostic markers for osteosarcoma.
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KEYWORD
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